Journal of Medical Pharmaceutical and Allied Sciences

  HPTLC method is developed to estimate gallic acid in the Poly herbal formulation. Developed methods were statistically evaluated according to ICH guidelines. Ethyl Acetate: Toluene: Methanol: Formic acid (3:2:1:5 v/v) was selected as a solvent system for HPTLC. The formulation was prepared by mixing all the ingredients in equal ratios and extracting them with Alcohol, Petroleum ether, Ethyl acetate, and chloroform. Pure Gallic acid is given a strong spot at Rf 0.65 in this solvent system and absorption maxima comes out to be 254 nm. Ethanolic extract of Salacia reticulata (EESR) and Ethanolic Extract of local formulation (EELF) showed spot of Gallic acid at Rf 0.65 along with nine other spots in this solvent system. The standard curve was plotted between different concentrations of Gallic acid and peak area. The method was found to be linear in the concentration range of 200-2000ng with a correlation coefficient of 0.985. In precision studies, % RSD (Relative standard deviation) ...

  Dry eye disease (DED) is a disorder of the ocular surface caused due to hyperosmolarity and insufficient production of tears to moisturize the eye, which leads to less lubrication and ocular surface inflammation and results in visual disturbance and discomfort to the eye. Long-time use of computers, laptops, and mobiles causes less blinking, making the eye's mucin layer thinner, leading to tear-film instability and DED. Tens of millions of people are getting affected by DED worldwide. It is also known as Sjogren's syndrome, which impacts the lacrimal gland of the eyes, decreases tear secretion, and causes the development of aqueous tear film in the eyes and meibomian gland dysfunction. Here we have discussed the various diagnosis methods used for identifying DED and also the emerging technologies for the diagnosis of DED. The treatment options are discussed as per the disease's severity, including novel therapies like the autologous serum, topical corticosteroids, and ant...

  The world is fighting against coronavirus disease 2019. Cancer patients are highly immunocompromised by cancer treatment. The conditions potentially increase the mortality and morbidity of head and neck cancer patients. This study aims to identify head and neck cancer prevalence in the Department of Otorhinolaryngology-Head and Neck Surgery Hasan Sadikin General Hospital, Bandung, from the intended year before and during the pandemic. Data were obtained from the hospital database of all head and neck cancer patients from 2018-to 2020. Head and neck cancer patients were compared between 2018-2020 based on outpatients, inpatients, and treatment using the Wilcoxon test to analyze the association between variables. There was a significant decrease in the number of patients and treatment in Hasan Sadikin General Hospital during the pandemic. This study showed a significant decline in patients, treatments, and procedures during the head and neck cancer pandemic. During this crisis and ...

  The sources of scientific literature on the research have been investigated, various approaches to the processing and modification of polymers have been analyzed, and the main direction of the study has been identified. The changes in the properties of thin layers of industrially produced low-density polyethylene (LDPE) grades 10803-20 and 16603-01operating01 operating under different climatic conditions have been studied having, and it has been determined that changes with different intensities occur in the structure and in this connection in the physical and mechanical properties of LDPE that was in operation at different time intervals and in different climatic conditions of the Absheron Peninsula and the Aran region: melt flow index of polymer alloy, tensile strength at break, relative extension, and values of the indicator sreredeteriorate ing. sreredeteriorate. Inpolymerm,samplareasis formed 24,2 – 28,6% of the insoluble phase and as a result of oxidation up to  12,3 – 16...

  Shoulder pain is a common complaint, with prevalence studies showing that 7–20 percent of the adult Indian population suffers from it. Functional disability is one of the most common complaints for patients with shoulder pain. The aim of shoulder pain treatment is generally to reduce pain and strengthen functional disabilities. So, this study will be conducted to compare the efficacy of Spencer technique, Kaltenborn, Mulligan, and Maitland mobilization in frozen shoulder. A total of 80 samples will be included and they will be divided into four group 20 in each. They will receive treatment 5 times a week for 3 weeks. On completion of the treatment in the third week, post interventional assessment and scores of VAS, ROM, and SPADI will be revaluated and follow-up and assessment of these outcomes will be revaluated on completion of 2nd month, 3rd month and 6th month and the data will be taken for analysis. Result will be drawn post the conduction of study. https://jmpas.com/abstrac...

  The objective of this study was to assess the drinking water quality from North, Central, South, and West regions of Chennai. The samples for analysis included metro water, ground water, Reverse Osmosis water, packaged drinking water, mineral water, boiled water, and rainwater. Analysis of sixteen physicochemical parameters such as pH, turbidity, Electrical conductivity, Total dissolved solids, Total suspended solids, major cations like calcium (Ca2+), sodium (Na+), and potassium (K+) and anions like chloride (Cl-), fluoride (F), nitrate (NO3-) and sulphate (SO42-) and heavy metals such as Copper (Cu), Zinc (Zn2+), magnesium (Mg), and Iron (Fe) are all measured using American Public Health Association (APHA)standard methods of analysis. The results were compared with standard values established by agencies such as World Health Organization (WHO), the Bureau of Indian Standards (BIS) and National Drinking Water Quality Standard (NDWQS). The Water Quality Index ranged from 11.66 to...

  A new compound of dihydropyrimidine derivatives was designed and predicted to have antibacterial effect. Synthesis of dihydropyrimidine derivatives could be carried out by reaction between Chalcone and thiourea to form dihydropyrimidine. It is incorporated with benzimidazole heterocycle. This study evaluated the mechanism of dihydropyrimidine derivatives in inhibition of DNAG with molecular docking. Docking was performed on the receptor file DNAG (PDB ID: 4DUH) using Auto Dock 1.5.6 program and visualized by Discovery Studio. The docking score of ligand standard, ciprofloxacin and dihydropyrimidine derivatives APUS17, APUS 20, APUS 9, APUS14 towards 4DUH were -8.0, -8.12, -12.1,-11.82, -10.96,-10.1 Kcal/mol respectively. From that derivatives having electron withdrawing group show highest binding affinity and having electron donating group show moderate binding ffinity. https://jmpas.com/abstract/957

  The aim of this research work was to develop a simple, accurate, sensitive and validated Ultra Violet (UV) spectrophotometric assay using the multivariate regression method for the analysis of Cilnidipine. This multivariate calibration technique was based on equations constructed using linear regression analysis using the correlation between absorbance and concentration at five selected equidistant wavelengths. Cilnidipine had a maximum absorbance at 240 nm. The findings were statistically analyzed for significance. A linear plot in the concentration range of 3-9 μg/mL, with a regression coefficient of 0.999 was obtained. The % RSD for intra-day and Inter-day precision were 0.4558 and 0.6099, respectively. The assay was determined and found to be 99.1% - 101.67% % w/w. https://jmpas.com/abstract/956

  Condensation of 1-hydroxycyclohexyl phenyl ketone with hydrazine carbothioamide produced a new Schiff base, 2-(1-hydroxycyclohexyl) phenyl methylene hydrazine carbothioamide. The Cu(II) and Zn(II) metal complexes of the Schiff base were prepared. The different spectroscopic methods such as FT-IR, UV-Visible, 1H NMR, and 13C NMR spectroscopy were used to elucidate the structural characteristics of the synthesized compounds. Further, the biological applications of the prepared Schiff bases and its metal complexes were studied for the antibacterial activity. The antimicrobial investigation was carried out against different Gram positive and Gram negative bacteria like Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. According to the findings, the prepared Cu(II) and Zn(II) metal complexes are more biologically active than Schiff base ligand, and among all tested bacterial species, Staphylococcus aureus growth was most inhibited by prepared compound...

  In recent years, many infectious veterinary diseases have caused massive losses in India's poultry population. These illnesses have a substantial impact on mortality, morbidity, and case fatality rates, resulting in large economic losses, as well as decreased productivity in the chicken industry. Infectious bursal disease (IBD) is sometimes called Gumboro disease. It is regarded as a highly infectious and epidemic disease that mainly spreads among poultry, turkeys, and wild birds through a virus. IBD is considered in the top 5 infectious diseases affecting chickens and birds which is a huge global threat and has caused many economic losses and welfare concerns. Direct impact on mortality from 3% to 30�pends upon the degree of protection of the birds and the form of the disease. The present review discusses about structure, serotypes, hosts & route and diagnosis of IBD and also collects information regarding surveillance and genomic analysis of IBD. https://jmpas.com/abstract/...

  Congenital Heart Disease is a condition present since birth having malformed heart structure and function. The malfunction of the heart hemodynamics requires palliative, supportive and corrective surgery. Children with congenital heart disease have compromised physiological function compared to normal with the same age and anthropometric measures. The energy consumption for a given submaximal work would be more in CHD patients compared to normal. The physiologic cost index calculated the energy expenditure and gives an idea about the same during walking. PCI can be used as an outcome measure to plan and tailor a rehabilitation exercise program. So the present observational study calculates the energy expenditure in repaired congenital heart disease patient and provides the baseline for a goal to achieve during any rehabilitation program by comparing with the normal. To compare the physiological cost index (PCI) between repaired congenital heart disease patients and healthy childr...

  The aim of the study was to fabricate and evaluate drug loaded nanosponges for ophthalmic drug delivery in a controlled manner for effective therapy. The nanosponges were formed by anionic polymerization of Besifloxacin hydrochloride with ethyl cellulose as a polymer, in presence of polyvinyl alcohol as surfactant. Besifloxacin HCl loaded ethyl cellulose nanosponges were formulated at different rpm i.e 1000 ,1200, 1500, 1800,2000,3000 & 3500 to check the best outcomes. This change in rpm results in different particle size, entrapment efficiency and in vitro release. The selected formulations were evaluated for their antibacterial effect and due to the sustained property of polymer and surfactant property of polyvinyl alcohol, formulated nanosponges showed enhanced antimicrobial study. https://jmpas.com/abstract/953

  For a long time, numerous attempts are being made by researchers to discover and develop new antimicrobial agents based on synthetic compounds and medicinal plants. These attempts were forced due to increasing rate of microbial resistance. In the present study, it has been discussed that the synthesis of various dihydropyrimidine fused with benzimidazole moiety. In which o-phenylenediamine and chloroacetic acid react in acidic medium by nucleophilic addition reaction to form 2-chloro methyl Benzimidazole. (1). The substituted Chalcone (2) was synthesized by a claisan-schmidt condensation reaction. The condensation of an aromatic aldehyde with aromatic ketone having α- hydrogen in presence of a strong base to form α,β-unsaturated ketone i.e Chalcone is form. The substituted Chalcone react with thiourea by Michael reaction. It is also called 1,4 addition reaction. In which thiourea act as nucleophile attack on 4-positon of the α,β-unsaturated ketone and keto-enol tautomerism occurs...

  Several anticancer drugs are getting resisted by the cancer cell and treatment like chemotherapy, radiation causes serious side effects. In immunomodulatory treatment the efficiency is less and CAR-T cells, CAR-NK cells require enormous time to get adopt to the in vitro and may cause seizures, dilemma, concussion in prolonged use against the cancer. Even the production of CAR-T cells and NK cells are tedious process. To overcome this situation, anticancer peptides can be used, as they don’t have any drug resistance and they can be highly potent, with good cell penetration. The advantages of these peptides are easy to modify, produce and formulate. This pandemic showed us that, identifying and characterizing a novel anticancer peptide (ACP) is an extremely time and labor consuming process. To reduce the time and labor, this study uses several in silico tools and algorithms like SVM, RF, XGBoost and KNN to predict a novel anticancer peptide. After several studies, with the collecte...

  We aim to explore the relationship between work pressure and depression, and sexual desire in a healthcare professional. A cross-sectional study was conducted with healthcare professionals between the age of 18-45 years in a multispecialty healthcare institute using the Hamilton Depression Rating scale (HAMD), and Telford and Wrekin sexual health questionnaire. In total, 150 people participated in our study, with a mean age of 28.83 ± 5.56 years. Among them22.0% of respondents had low work pressure, 42.7% had moderate work pressure, and 35.3% had heavy work pressure. In terms of sexual desire, 20.7% (31) of the participants desired to have sexual relations every day, 22% (33) twice a week, 22% (33) once a week, 15.3% (23) once a month, and 20% (30) of the participants did not have sexual desire. As per the HAM-D scale assessment, 40.7% of the participants were normal, while 12.7 % had mild depression. People who were moderately, severely, or highly depressed made-up 16%, 10.7%, a...

  This study was conducted to investigate the effects of unaffected-side active treatment on the gait of hemiplegic patients. The study subjects were 20 patients who showed hemiplegic symptoms after a stroke. Through a random classification method, the subjects were divided into an experimental group (n = 10) who underwent treadmill exercise based on the concept of unaffected-side active treatment to strengthen the paretic side lower extremity after proprioceptive neuromuscular facilitation (PNF) treatment and a control group (n = 10) who underwent general treadmill training without unaffected side-active treatment after PNF treatment. The therapeutic interventions were performed three times a week for 4 weeks. To analyze behavioural motor motions, the patient's gait speeds and ankle angles were measured using the timed up-and-go (TUG) and the Dartfish program before treatment and 14 and 28 days after beginning treatment. The study results showed that the TUG and ankle angles signi...

  Chronic obstructive pulmonary disease is a significant state that leads to progressive airflow obstruction and subsequent irreversible damage to the airways. It is a major factor causing death and has a very high mortality rate worldwide. In recent years, the mortality rate has increased due to Chronic obstructive pulmonary disease (COPD) and it is estimated to increase in the coming years. This paper reviews the emerging techniques using these technologies that can be used to detect and monitor the severity of chronic obstructive pulmonary disease. The Internet of Things can help to detect and monitor the condition of a patient suffering from chronic obstructive pulmonary disease using sensors which are used to measure a particular parameter like concentration of different gases present in the exhaled breath and ensure that his condition doesn’t get worse. Using an Artificial Intelligence and Machine Learning based approach, a system can be developed where the data is collected ...

  Biological alterations result in unchecked cell growth and division producing cancer. Mankind has always been interested in light, and, it being both a topic of study and a device for looking into other occurrences can be seen following the inception of time. Besides the development of nanosystems, the usage of light has now entered a new level where interactions between light and matter occur at wavelength and subwavelength levels accompanying management by physico-chemical properties of nanoforms. This area of nanophotonics enables the investigation and control of light-encompassing nanoforms, individual molecules, and biomolecular assemblies. Numerous molecular cancer treatments have been developed using the amazing nanoscale features as a result of nanophotonics in biomolecular interactivities, or nanobiophotonics. In this work, we demonstrate the applications of nanobiophotonics and of multifaceted nanoplatforms which constitute excellent treatment efficiency and impart brea...

  The main aim of this study is to develop a rapid and cheap Ultraviolet (UV) spectrophotometric method for the estimation of Favipiravir (400mg) in tablets and validate as per International Conference on Harmonization (ICH) guidelines. The developed method uses the solvent Ethanol: Water (30:70) for the assay of Favipiravir whose λmax was found to be 231nm. In this method, Favipiravirshows linearity in the range 2-10 μg/ml. The accuracy was carried out by using standard addition method and the precision is ascertained by calculating the relative standard deviation. A cheap and rapid UV spectrophotometric method was developed and validated for the estimation of Favipiravir and hence it can be used for the routine pharmaceutical analysis. https://jmpas.com/abstract/1138

  Georgia is one of the world's oldest wine areas. Interest to by-products of vine processing is rising because they may be exploited as a source of bioactive compounds for pharmaceutical and cosmetic purposes, rather than being discarded. The aim of the present research was to study polyphenolic compounds content in Saperavi grapevine shoots extract. Shoots of Saperavi grapevine were collected in Kakheti, Georgia. Extract was preliminary prepared and concentrated 4-fold. Polyphenols were extracted using Methanol and total phenolic content (TPC) was measured with Folin-Ciocalteu (F-C) reagent in terms of Gallic acid. The qualitative composition and content of phenolic compounds was studied with Liquid Chromatography - tandem Mass Spectrometry (LC–MS/MS). The identification was achieved by comparison of the retention times (tR) and spectra characteristics of individual compounds with those in data library. As a result, 14 different phenolic compounds were isolated. Received results ...

  Currently, one of the most challenging diseases to tis breast cancer. Hormone therapy, surgery, chemotherapy, and radiation have all been used to treat breast cancer for a long time, but they are become eating less and less successful due to serious side effects and rising drug resistance. Numerous dietary foods have been linked to altered cancer genesis and progression pathways, decreased aggressiveness, and growth inhibition of malignant cells. These dietary foods may be advantageous compared to produce medications since they exhibit lower toxicity and negative effects in various investigations. The promises made by dietary foods for breast cancer treatment by focusing on several pathophysiological cascades involved in the onset and progression of breast cancer and their capacity to simultaneously stop metastatic and growth progression. Some of the extensively researched numerous dietary foods that specifically target certain breast cancer pathways and express any of the three ...

  The disorder essentially inside the radio humeral joint with constant impairing pain in the elbow, is named as tendonitis, tennis elbow, lateral epicondylalgia or lateral epicondylitis. The precise explanation for tendonitis is not however, identified. It is quite common in people whose jobs demand recurring forearm movements (e.g., court game sportsmen and woodworkers). It is normally because of additional fast, wearisome, recurrent eccentric contractions and gliding joint griping activities. It normally affects dominant arm. The typical amount of associate degree episode of epicondylitis in span of six months and two years. In tennis elbow macroscopic and microscopic lesions appears within the Extensor Carpi Radialis Brevis (ECRB) muscle. Effectiveness of the interventions on the pain and the functional improvement was assessed by VAS (visual analogue scale) and the Tennis Elbow Function Scale (TEFS) respectively. The findings of the study will greatly contribute for the eviden...

  In the present study the function of anterior chamber depth (ACD) changes in determining the refractive outcome after cataract surgery has been studied comprehensively. 210 successive adult patients going through unilateral cataract surgery (phacoemulsification by a single surgeon) were included in this prospective study. Depending on axial length patients were suitably classified into 3 groups: Group 1: <22 xss=removed xss=removed>26 mm (n = 70). Pre-operative and post-operative ACD were assessed on immersion ultrasound. The patients were arbitrarily reclassified into group A (≤1.7 mm) and B (>1.7 mm) based on mean ACD change. Post-operative emmetropic, myopic, or hypermetropic status was also determined in all patients. Mean ACD change in short eyes (Group 1), normal (Group 2), and long (Group 3) eyes was 1.90 mm, 1.75 mm, and 1.45 mm, respectively. The Hyperopic shift was noted when the change in ACD was less than 1.70 mm and Myopic shift was noted when the alteration...

  Triple-negative breast cancer (TNBC) is considered to be more aggressive and have a poorer prognosis than other types of breast cancer. The specific gene expression pattern between TNBC and other non-triple negative breast cancer (nTNBC) is yet to be resolved. Hence the present study was aimed to study the BRCA-1 status and measure the important tumor markers mRNA expression in peripheral blood samples of TNBC and other nTNBC subjects and compared with control subjects. EDTA blood samples were obtained from 25 CA (cancer) breast patients and 25 controls for DNA and RNA isolation. PCR-RFLP analysis was performed to check the BRCA-1 mutation in all the samples. Results showed that BRCA-1 mutation was present in all TNBC patients. Subsequently, quantitative RT-PCR was performed to detect the relative mRNA expression of NF-κβ, VEGFA, Caspase-3, and ERBB2 gene transcripts. Results showed that the transcript levels of NF-κβ, VEGFA, and ERBB2 were significantly up regulated in TNBC (p=0...